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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
24/03/2008 |
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Data da última atualização: |
22/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. |
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Afiliação: |
Felipe Perecin, USP/Pirassununga; SIMONE CRISTINA MEO NICIURA, CPPSE; Walt Yamazaki, Biotecnologia da Reprodução Animal Ltda; Christina Ramires Ferreira, Unicamp; Fernando Henrique Biase, USP/Pirassununga; Giovana Krentel Merighe, USP/Pirassununga; Flávio V. Meirelles, USP/Pirassununga; Joaquim Mansano Garcia, UNESP/Jaboticabal. |
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Título: |
Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Some gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed reallocation randomization test (Pfaffl et al. 2002 Nucl. Acids Res. 30, e36) was used. All expression ratios were normalized by glyceraldehyde 3-phosphate dehydrogenase expression and calibrated by the in vivo group (expression assumed as 1.00 for all genes and tissues). The analysis of relative differences on transcript levels of imprinted genes in fetuses revealed IGF2 down-regulation (P < 0.05) in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to the in vivo group and IVF fetuses (2.02). In chorioallantois, IGF2 was down-regulated (P < 0.001) in parthenotes (0.001) when compared to the in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was down-regulated (P < 0.001) in SCNT chorioallantois (0.25) when compared to the in vivo group. Low expression of IGF2 in parthenogenetic fetuses and chorioallantois confirms its imprinted status in bovine. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in bovine SCNT-derived fetuses and chorioallantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes for the low efficiency of SCNT procedures in this species. Such alterations suggest modifications in DNA methylation patterns at IGF2 and IGF2R imprinting centers. MenosSome gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed rea... Mostrar Tudo |
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Palavras-Chave: |
In vivo-and in vitro; Placentas; Produced bovine. |
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Thesaurus Nal: |
gene expression. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42088/1/PROCI-2008.00004.pdf
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Marc: |
LEADER 03522nam a2200241 a 4500
001 1048224
005 2023-06-22
008 2008 bl uuuu u00u1 u #d
100 1 $aPERECIN, F.
245 $aImprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas.$h[electronic resource]
260 $aReproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.$c2008
520 $aSome gestational alterations associated with bovine somatic cell nuclear transfer (SCNT) are presumably consequences of abnormal imprinted gene expression. This work aimed to evaluate the expression patterns of imprinted genes IGF2 and IGF2R in bovine fetuses and chorioallantoic membranes derived from in vivo- and in vitro-produced embryos. Fetuses were produced by AI (in vivo group, n = 3), IVF (n = 3), parthenogenesis (n = 3), or SCNT (n = 2). Cows with positive pregnancy diagnosis after ultrasonographic examination were slaughtered between Days 33 and 36 of gestation. The reproductive tract was transported on ice to the laboratory, where fetuses and chorioallantoic fragments were collected and stored in liquid nitrogen. Total RNA extraction was performed using TRIzol, according to manufacturer's instructions, and the reverse transcription reaction was carried out with 1 µg of total RNA, 6.75 µm oligo pd(T)12?18, and 50 U of reverse transcriptase (Improm-II, Promega, Madison, WI, USA). The relative quantification of IGF2 and IGF2R transcripts was done using real-time PCR with SYBR Green dye. The average efficiency of PCR amplifications was estimated for each gene using a linear regression on the logarithm of fluorescence per cycle (Ramakers et al. 2003 Neurosci. Lett. 339, 62?66), and the expression ratios were calculated according to the method described previously by Livak and Schmittgen (2001 Methods 25, 402?408). To verify statistical differences, a pair-wise fixed reallocation randomization test (Pfaffl et al. 2002 Nucl. Acids Res. 30, e36) was used. All expression ratios were normalized by glyceraldehyde 3-phosphate dehydrogenase expression and calibrated by the in vivo group (expression assumed as 1.00 for all genes and tissues). The analysis of relative differences on transcript levels of imprinted genes in fetuses revealed IGF2 down-regulation (P < 0.05) in the SCNT (0.19) and parthenogenetic (0.02) groups when compared to the in vivo group and IVF fetuses (2.02). In chorioallantois, IGF2 was down-regulated (P < 0.001) in parthenotes (0.001) when compared to the in vivo, IVF (3.13), and SCNT (0.98) groups. IGF2R was down-regulated (P < 0.001) in SCNT chorioallantois (0.25) when compared to the in vivo group. Low expression of IGF2 in parthenogenetic fetuses and chorioallantois confirms its imprinted status in bovine. Alterations in the relative frequency of IGF2 and IGF2R transcripts were observed in bovine SCNT-derived fetuses and chorioallantoic membranes, respectively, supporting the hypothesis that abnormalities in the expression of imprinted genes are causes for the low efficiency of SCNT procedures in this species. Such alterations suggest modifications in DNA methylation patterns at IGF2 and IGF2R imprinting centers.
650 $agene expression
653 $aIn vivo-and in vitro
653 $aPlacentas
653 $aProduced bovine
700 1 $aNICIURA, S. C. M.
700 1 $aYAMAZAKI, W.
700 1 $aFERREIRA, C. R.
700 1 $aBIASE, F. H.
700 1 $aMERIGHE, G. K. F.
700 1 $aMEIRELLES, F. V.
700 1 $aGARCIA, J. M.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 24 | |
| 1. |  | NICIURA, S. C. M.; PERECIN, F.; SARAIVA, N. Z. Controle epigenético do desenvolvimento e do envelhecimento em mamíferos. In: NICIURA, S. C. M.; SARAIVA, N. Z. (Ed.). Epigenética: bases moleculares, efeitos na fisiologia e na patologia, e implicações para a produção animal e a vegetal. Brasília, DF: Embrapa, 2014. Cap. 7, p. 155-187.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|    |
| 3. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 4. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; VANTINI, R.; GARCIA, J. M. Efeitos da demecolcina sobre a cinética da maturação nuclear e a migração dos grânulos corticais em oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1276, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1276.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 5. | | MÉO, S. C.; YAMAZAKI, W.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; LEAL, C. L. V.; GARCIA, J. M. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments. Zygote, v. 15, p. 295-306, nov. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. | | LANDIM JR, L. P.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; MIGLINO, M. A.; GARCIA, J. M. Análise da expressao gênica do VEGF (Vascular Endothelial Growth Factor) em placentas bovinas de animais clonados, produzidos in vitro e in vivo, pelo método de RT-PCR semiquantitativo. ARS VETERINARIA, v. 22, n. 3, p. 217-222, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | DEL COLLADO, M.; SARAIVA, N. Z.; LOPES, F. L.; CRUZ, M. H.; GASPAR, R. C.; OLIVEIRA, C. S.; PERECIN, F.; GARCIA, J. M. Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos. Pesquisa Veterinária Brasileira v. 34, n. 7, p. 689-694, jul. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 8. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M. Effects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes. Reproduction, Fertility and Development, v. 20, n. 1, p. 107-108, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; OLIVEIRA, C. S.; MONTEIRO, F. M.; LIMA, M. R.; NICIURA, S. C. M.; FERREIRA, C. R.; GARCIA, J. M. Effects of embryonic fluid and serum replacer as protein sources for in vitro maturation of bovine oocytes. In: INTERNATIONAL SYMPOSIUM ANIMAL BIOLOGY OF REPRODUCTIVE, 3., 2010, Águas de São Pedro: CBRA: USP/FMVZ, 2010| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | NICIURA, S. C. M.; FERREIRA, C. R.; PERECIN, F.; YAMAZAKI, W.; LEAL, C. L. M.; GARCIA, J. M.; MEIRELLES, F. V. Desenvolvimento embrionário, visualização de pronúcleos e transferência pronuclear após centrifugação de zigotos bovinos em meio com citocalasina. São Carlos, SP: Embrapa Pecuária Sudeste, 2007 34 p. (Embrapa Pecuária Sudeste, Boletim de pesquisa e desenvolvimento,11). ISSN 1981-2078| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 12. | | MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 13. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Genetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 15. | | FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 16. | | FERREIRA, C. R.; MEIRELLES, F. V.; YAMAZAKI, W.; CHIARATTI, M. R.; NICIURA, S. C. M.; PERECIN, F.; SMITH, L. C.; GARCIA, J. M. The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos. Cloning and Stem Cells. v. 9, n. 4, p. 618-629, dec. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 17. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERREIRA, C. R.; MÉO, S. C.; OLIVEIRA, C. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Avaliação da influência da ovalbumina (OVA) como suplemento protéico durante a etapa de fecundação in vitro de oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERRERIA, C. R.; NICIURA, S. C. M.; OLIVEIRA, C. MELO, D. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Avaliação da qualidade em embriões PIV com a substituição do soro fetal bovino e da albumina sérica bovina pela ovalbumina. Acta Scientiae Veterinariae, v. 36, (Supl. 2), 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 19. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERREIRA, C. R.; MÉO, S. C.; OLIVEIRA, C. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Efeitos da substituição do soro fetal bovino (SFB) e da albumina sérica bovina (BSA) pela ovalbumina (OVA) na produção in vitro de embriões bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | PERECIN, F.; YAMAZAKI, W.; FERREIRA, C. R.; MÉO, S. C.; BIASE, F. H.; MERIGHE, G. K. F.; SARAIVA, N. Z.; TETZNER, T. A. D.; MEIRELLES, F. V.; GARCIA, J. M. Expressão de DNA metiltransferases em blastocistos bovinos produzidos in vivo e in vitro. Acta Scientiae Veterinariae, v. 35, supl. 3, p. 1181, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 24 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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